Work

Cytotoxicity evaluation of microbial sophorolipids and glucolipids using normal human dermal fibroblasts (NHDF) in vitro

Year: 2024

Type: article

Abstract: -C18:1, SL-C18:0) to study their potential to promote wound healing by measuring the gene expression of several key skin regeneration markers (i.e. collagen, elastin, transforming growth factor β, fibroblast growth factor …) using qPCR. Unfortunately, none of these glycolipids modulated the gene expression of molecules involved in tissue repair. However, this study aims to encourage the community to test this novel class of molecules for novel high-end biomedical applications. Importance: Biosurfactants prepared by microbial fermentation are natural amphiphiles of growing importance, with the goal of replacing synthetic surfactants in commercial formulations. However, their cytotoxicity profile is still poorly known, especially for new molecules like single-glucose lipids or bolaform sophorolipids. This wants to contribute to all those applications, which could be developed with biosurfactants in contact with the skin (cosmetics, wound healing). We test the cytotoxicity of five structurally-related molecules (C18:1 and C18:0 sophorolipids, C18:1 and C18:0 single-glucose lipids, C18:1 di-sophoroside) against normal human dermal fibroblasts (NHDF) and evaluate the metabolic activity of the least toxic among them. To the best of our knowledge, cytotoxicity of these molecules, and of microbial biosurfactants in general, was never tested against NHDF.

Source: Toxicology Reports

DOI: 10.1016/j.toxrep.2024.101862

Authors: Sergio Oliveira Formoso, Vincent Chaleix, Niki Baccile, Christophe Helary

Institutions: Centre National de la Recherche Scientifique, Sorbonne Université, Chimie de la Matière Condensée de Paris, Laboratoire des Agroressources, Biomolécules et Chimie pour l’Innovation en Santé, Université de Limoges

Language: English

Topic: Microbial bioremediation and biosurfactants

Subfield: Pollution

Field: Environmental Science

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,230