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Investigations into the growth and formation of biofilm by Leptospira biflexa at temperatures encountered during infection

Year: 2024
Type: article
Abstract: had more biomass compared to 29 °C, as determined by crystal violet staining. Confocal microscopy determined that the protein content within the biofilm matrix was more prominent than double-stranded DNA, and featured a distinct layer attached to the solid substrate. Additionally, the model enabled effective protein extraction for proteomic comparison across different biofilm phenotypes. Results highlight an important role for proteins in biofilm matrix structure by leptospires and the identification of their specific protein components holds promise for strategies to mitigate biofilm formation.
Source: Biofilm
DOI: 10.1016/j.bioflm.2024.100243
Authors: Priscyla dos Santos Ribeiro, Judith Stasko, Adrienne Shircliff, Luis Guilherme Fernandes, Ellie J. Putz, Claire Andreasen, Vasco Azevedo, Paula Ristow, Jarlath E. Nally
Institutions: Universidade Federal de Minas Gerais, Universidade Federal da Bahia, Agricultural Research Service, United States Department of Agriculture, National Animal Disease Center, Iowa State University
Language: English
Open Access status: gold
Estimated APC paid: $2,770
OpenAlex ID: W4405220304
FWCI: 0.693
Cites: 55
Cited by: 6
Related to: 10