Blood-based quantification of Aβ oligomers indicates impaired clearance from brain in ApoE ε4 positive subjects
Year: 2024
Type: article
Abstract: BACKGROUND: Quantification of Amyloid beta (Aβ) oligomers in plasma enables early diagnosis of Alzheimer's Disease (AD) and improves our understanding of underlying pathologies. However, quantification necessitates an extremely sensitive and selective technology because of very low Aβ oligomer concentrations and possible interference from matrix components. METHODS: In this report, we developed and validated a surface-based fluorescence distribution analysis (sFIDA) assay for quantification of Aβ oligomers in plasma. RESULTS: The blood-based sFIDA assay delivers a sensitivity of 1.8 fM, an inter- and intra-assay variation below 20% for oligomer calibration standards and no interference with matrix components. Quantification of Aβ oligomers in 359 plasma samples from the DELCODE cohort reveals lower oligomer concentrations in subjective cognitive decline and AD patients than healthy Control participants. CONCLUSIONS: Correlation analysis between CSF and plasma oligomer concentrations indicates an impaired clearance of Aβ oligomers that is dependent on the ApoE ε4 status.
Source: Communications Medicine
Authors: Lara Blömeke, Fabian Rehn, Marlene Pils, Victoria Kraemer-Schulien, Anneliese Cousin, Janine Kutzsche, Tuyen Bujnicki, Silka D. Freiesleben, Luisa-Sophie Schneider, Lukas Preis, Josef Priller, Eike J. Spruth, Slawek Altenstein, Anja Schneider, Klaus Fliessbach, Jens Wiltfang, Niels Hansen, Ayda Rostamzadeh, Emrah Düzel, Wenzel Glanz, Enise I. Incesoy, Katharina Buerger, Daniel Janowitz, Michael Ewers, Robert Perneczky, Boris-Stephan Rauchmann, Stefan Teipel, Ingo Kilimann, Christoph Laske, Matthias H. Munk, Annika Spottke, Nina Roy, Michael T. Heneka, Frederic Brosseron, Michael Wagner, Sandra Roeske, Alfredo Ramirez, Matthias Schmid, Frank Jessen, Oliver Bannach, Oliver Peters, Dieter Willbold
Institutions: Forschungszentrum Jülich, German Center for Neurodegenerative Diseases, Humboldt-Universität zu Berlin, Freie Universität Berlin, Charité - Universitätsmedizin Berlin, UK Dementia Research Institute, Technical University of Munich, University of Edinburgh, University of Bonn, Universitätsmedizin Göttingen, University of Aveiro, University of Göttingen, University of Cologne, Otto-von-Guericke-Universität Magdeburg, University Hospital Magdeburg, Institut für Schlaganfall- und Demenzforschung, Ludwig-Maximilians-Universität München, LMU Klinikum, Munich Cluster for Systems Neurology, Imperial College London, University of Sheffield, Universitätsmedizin Rostock, Hertie Institute for Clinical Brain Research, University of Tübingen, University Hospital Bonn, University of Luxembourg, Luxembourg Centre for Systems Biomedicine, Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases, University Hospital Cologne, Heinrich Heine University Düsseldorf
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,490
Award: G1858960198
OpenAlex ID: W4405221727