Work

Pod5Viewer: a GUI for inspecting raw nanopore sequencing data

Year: 2024

Type: article

Abstract: MOTIVATION: Oxford Nanopore Technologies recently adopted the POD5 file format for storing raw nanopore sequencing data. The information stored in these files provides detailed insights into the sequencing features and enhances the understanding of raw nanopore data. However, the process of visualizing the data can be cumbersome, especially for users without programming skills. To address this issue, we developed the pod5Viewer, a GUI application for inspecting POD5 files. RESULTS: The pod5Viewer offers straightforward access to raw sequencing data and associated metadata in POD5 files. It includes functionalities for viewing, plotting, and exporting individual reads. Designed with user-friendliness in mind, the pod5Viewer is easy to install and use, making it suitable for users with all technical backgrounds. AVAILABILITY AND IMPLEMENTATION: The pod5Viewer is available as open source from the pod5Viewer Github repository (https://github.com/dietvin/pod5Viewer).

Source: Bioinformatics

DOI: 10.1093/bioinformatics/btae665

Authors: Vincent Dietrich, Nicolò Alagna, Mark Helm, Susanne Gerber, Tamer Butto

Institutions: Johannes Gutenberg University Mainz, University Medical Center of the Johannes Gutenberg University Mainz

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $4,188

Funder: Deutsche Forschungsgemeinschaft