Development and Characterization of a Lysine Biosensor using Lysine Oxidase Nanoparticles for Lysine Detection using Pencil Graphite Electrode
Year: 2024
Type: article
Abstract: The precise detection of lysine is critical in clinical diagnostics and food safety due to its role as an essential amino acid and a biomarker in various physiological processes (Nivedita et al., 2019; Sarma & Choudhury, 2016). This study presents the development and characterization of a high-performance lysine biosensor based on lysine oxidase nanoparticles immobilized on pencil graphite electrodes (PGEs). Advanced characterization techniques, including Fourier-transform infrared spectroscopy (FTIR), UV-visible spectroscopy (UV-Vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and electrochemical analysis (cyclic voltammetry, electrochemical impedance spectroscopy, and amperometry), were employed to validate the sensor's design and performance (Chen & Qian, 2018; Liao & Xiao, 2016).The biosensor demonstrated a remarkable limit of detection (LOD) of 0.1 µM and a wide linear detection range spanning 0.1 µM to 1.0 mM, covering physiological and supra-physiological lysine levels (Duffy & Gillis, 2015). pH optimization revealed maximum enzymatic activity at pH 6.5, while stability tests confirmed that the sensor retained over 95% of its activity after 30 days of storage (Sharma & Byrne, 2016). The integration of lysine oxidase with a nanoparticle-modified electrode significantly enhanced sensitivity (8.2 µA/mM) and specificity, outperforming existing lysine detection technologies (Rodriguez & Fernandez, 2020).These findings establish the proposed biosensor as a cost-effective, scalable, and reliable platform for lysine monitoring in real-world applications, such as medical diagnostics and food quality control.
Source: Nanotechnology Perceptions
Authors: Neha Sawarni, Dr Parveen Kumar, Prof. Minakshi Sharma
Institution: Baba Mastnath University
Language: English
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4405233174