Get started
Work

Fibroblasts regulate the transcriptional signature of human papillomavirus-positive keratinocytes

Year: 2024
Type: article
Abstract: Persistent human papillomavirus (HPV) infection is necessary but insufficient for viral oncogenesis. Additional contributing co-factors, such as immune evasion and viral integration have been implicated in HPV-induced cancer progression. It is widely accepted that HPV + keratinocytes require co-culture with fibroblasts to maintain viral DNA as episomes. How fibroblasts regulate viral episome maintenance is a critical knowledge gap. Here we present comprehensive RNA sequencing and proteomic analysis demonstrating that coculture with fibroblasts is supportive of the viral life cycle, and is confirmatory of previous observations. Novel observations suggest that errors in "cross-talk" between fibroblasts and infected keratinocytes may regulate HPV integration and drive oncogenic progression. Our co-culture models offer new insights into HPV-related transformation mechanisms.
DOI: 10.1016/j.tvr.2024.200302
Authors: Claire D. James, Rachel L. Lewis, Austin J. Witt, Christiane Carter, Nabiha M. Rais, Xu Wang, Molly L. Bristol
Language: English
Subfield: Epidemiology
Field: Medicine
Open Access status: gold
Estimated APC paid: $2,400
Funders: U.S. Department of Health and Human Services, Virginia Commonwealth University, Meharry Medical College, National Institutes of Health, VCU Massey Cancer Center, National Cancer Institute, National Institute of Dental and Craniofacial Research
Awards: G1154943605, G2524726931, G2605286076, G2715011213, G4831788520, G49283514, G542644415, G5881522192, Estrogen reduces HPV transcription and oncogene expression to target disease, G8624700338, G8829921498, G9000186613, Massey Cancer Center - Cancer Center Support Grant
OpenAlex ID: W4405236353
FWCI: 1.558
Cites: 177
Cited by: 6
Related to: 10