Year: 2024
Type: article
Abstract: This work presents the development of an amperometric biosensor for detecting aspartate aminotransferase (AST) activity in biological fluids using a platinum disk electrode as the working transducer. Optimal concentrations of substrates (aspartate, α-ketoglutarate) and the coenzyme (pyridoxal phosphate) were determined to ensure efficient biosensor operation. A semi-permeable poly-m-phenylenediamine membrane was applied to enhance selectivity against electroactive interferents. The biosensor demonstrated good stability (storage, continuous operation, and production reproducibility) and analytical performance (sensitivity 8.56 nA/min for 50 U/L AST, LOD 1 U/L, linear range 1-110 U/L). Testing with real samples showed a high correlation (R = 0.989) with spectrophotometric analysis, supporting its potential for further applications.
Source: Sensors
DOI: 10.3390/s24247891
Institutions: National Academy of Sciences of Ukraine, Institute of Molecular Biology and Genetics, Taras Shevchenko National University of Kyiv, Ternopil Ivan Pului National Technical University, I.Horbachevsky Ternopil National Medical University, University of Bielsko-Biała, National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”
Language: English
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,955
OpenAlex ID: W4405237260