Comparative evaluation of apical microleakage of mineral trioxide aggregate, Biodentine, and Bio-C Repair as root-end filling materials using dye extraction method: An in vitro study
Year: 2024
Type: article
Abstract: Context: Root-end filling materials enhance the apical sealing ability of the root canal to avoid leakage of irritants and to prevent the reentering of microorganisms. Aim: The objective of the present study was to compare and evaluate apical microleakage in retrograde filling materials with mineral trioxide aggregate (MTA), Biodentine, and Bio-C Repair using an ultraviolet (UV)–visible spectrophotometer. Materials and Methods: Fifty maxillary incisors were selected and decoronated. Instrumentation was done with ProTaper rotary files and obturated with AH Plus sealer and ProTaper gutta-percha cone using lateral condensation technique. The apical part of each root was resected at 90° to the long axis of the root for 3 mm, and retrograde cavity preparation was done up to 3 mm using an ultrasonic retro tip. After conditioning the root end with 17% ethylenediaminetetraacetic acid, the teeth were divided into four groups. Group 1 = Positive Control ( n = 5), Group 2 was filled with MTA ( n = 15), Group 3 was filled with Biodentine ( n = 15), and Group 4 was filled with Bio-C Repair ( n = 15). All samples were incubated in 5 mL of 2% methylene blue dye for 72 h after which teeth were immersed in 65% nitric acid for 72 h. The solutions were then filtered using fine grit filter paper and centrifuged at 3500 rpm for 5 min. The solution thus collected was used to determine absorbency in UV–visible spectrophotometer at 550 nm. Statistical Analysis: The data were analyzed using one-way analysis of variance and Tukey post hoc tests. P <0.05 was considered significant for all analyses. Results: The results showed that Biodentine had the least dye absorbance, which means less microleakage, and there was no significant difference between MTA and Bio-C Repair. Conclusion: Biodentine showed superior sealing ability as a retrograde filling material compared to Bio-C Repair and MTA.
Authors: Divya Kunam, Yamuna Bode, Pavanila Meghana Narra, C. H. N. V. Murali Krishna, Satti Narayana Reddy, Modem Venkateshwarlu
Institution: Narayana Dental College and Hospital
Language: English
Subfield: Oral Surgery
Field: Dentistry
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W4405261244