Work

Optimal extraction of Chenopodium formosanum leaves using the response surface method

Year: 2024

Type: article

Abstract: Chenopodium formosanum (CF) is a species endemic to Taiwan that is rich in both nutrients and antioxidants. Although the seeds of CF have long been considered valuable sources of antioxidants, no studies have yet examined the levels of antioxidant in CF leaves or these leaves’ optimal extraction methods. In this study, Response Surface Methodology (RSM) was applied to determine the optimal conditions for promoting total phenolic content (TPC), total flavonoid content (TFC), di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) radical scavenging, 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging, and ferric reducing ability of plasma (FRAP). Results showed that the concentration of ethanol had a major effect on TPC, DPPH radical scavenging, ABTS radical scavenging, and FRAP, whereas temperature had a major effect on TFC. The final optimized results for 100 mL ethanol solution to extract 1 g CF leaves determined using RSM were 141.26 µg gallic acid equivalents/mL for TPC, 158.09 µg quercetin equivalents/mL for TFC, 92.85% for DPPH radical scavenging, 50.49% for ABTS radical scavenging, and 26.85 µg vitamin C equivalents/mL for FRAP. RSM employed in this study revealed that CF leaves, similar to CF seeds, are a potent source of antioxidants.

Source: Cogent Food & Agriculture

DOI: 10.1080/23311932.2024.2436643

Authors: Yung-Sheng Lin, Shiou-Yih Hu, Cheng-You Chen, Chin-Tung Wu, Wei-Hsun Wang

Institutions: National United University, National Yunlin University of Science and Technology, Chaoyang University of Technology, National Chung Hsing University, Changhua Christian Hospital

Language: English

Topic: Phytochemicals and Antioxidant Activities

Subfield: Biochemistry

Field: Medicine

Domain: Health Sciences

Open Access status: diamond

Funder: Ministry of Science and Technology, Taiwan

Awards: G2076257756, G5003207823