Work

New insights into the molecular architecture of neurons by cryo-electron tomography

Year: 2024

Type: review

Abstract: Cryo-electron tomography (cryo-ET) visualizes natively preserved cellular ultrastructure at molecular resolution. Recent developments in sample preparation workflows and image processing tools enable growing applications of cryo-ET in cellular neurobiology. As such, cryo-ET is beginning to unravel the in situ macromolecular organization of neurons using samples of increasing complexity. Here, we highlight advances in cryo-ET technology and review its recent use to study neuronal architecture and its alterations under disease conditions. • In situ cryo-ET provides novel mechanistic insights into neuronal biology. • Cryo-ET enables cellular structural studies of neurodegeneration. • Recent developments allow cryo-ET imaging of native nervous tissues.

Source: Current Opinion in Neurobiology

DOI: 10.1016/j.conb.2024.102939

Authors: Arsen Petrovic, Thanh Thao Do, Rubén Fernández-Busnadiego

Institutions: Universitätsmedizin Göttingen, Multiscale Bioimaging, University of Göttingen

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,480