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Rab10 inactivation promotes AMPAR insertion and spine enlargement during long-term potentiation

Year: 2024
Type: preprint
Abstract: Summary Rab-dependent membrane trafficking is critical for changing the structure and function of dendritic spines during synaptic plasticity. Here, we developed highly sensitive sensors to monitor Rab protein activity in single dendritic spines undergoing structural long-term potentiation (sLTP) in rodent organotypic hippocampal slices. During sLTP, Rab10 was persistently inactivated (>30 min) in the stimulated spines, whereas Rab4 was transiently activated over ∼5 min. Inhibiting or deleting Rab10 enhanced sLTP, electrophysiological LTP and AMPA receptor (AMPAR) insertion during sLTP. In contrast, disrupting Rab4 impaired sLTP only in the first few minutes, and decreased AMPAR insertion during sLTP. Thus, our results suggest that Rab10 and Rab4 oppositely regulate AMPAR trafficking during sLTP, and inactivation of Rab10 signaling facilitates the induction of LTP and associated spine structural plasticity.
Source: eLife
DOI: 10.7554/elife.103879.1
Authors: Jie Wang, Jun Nishiyama, Paula Parra-Bueno, Elwy Okaz, Goksu Oz, Xiaodan Liu, Tetsuya Watabe, Irena Suponitsky-Kroyter, Timothy E McGraw, Erzsebet M Szatmari, Ryohei Yasuda
Institutions: Duke University, Max Planck Florida Institute for Neuroscience, Duke Medical Center, Duke-NUS Medical School, Weill Cornell Medicine, East Carolina University
Language: English
Open Access status: gold
Estimated APC paid: $2,500
OpenAlex ID: W4405281382
Cites: 91
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Related to: 10