Protocol for in situ and in vivo dissection of mouse lung-draining lymph nodes
Year: 2024
Type: article
Abstract: Lung-draining lymph nodes (LNs) play crucial roles in infection, inflammation, and cancer, but in situ and in vivo studying models are technically challenging. Here, we present a protocol for the in situ and in vivo dissection of mouse lung-draining LNs, ensuring postoperative survival. We describe steps for lymphadenectomy including localizing lung-draining LNs, open thoracotomy, and LN dissection. We then detail procedures for surgical sample preservation and postoperative care. This protocol offers broad applications in immunological and oncological research.
Source: STAR Protocols
Authors: Enshuo Zhang, Baicheng Zhao, Yupeng Liu, Gang Liu, Shuting Lu, Liqin Xiong, Xufeng Pan, Haitang Yang, Feng Yao
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,500
Awards: G2216861808, G8840166811
OpenAlex ID: W4405289571