Antiviral-effect of nitrogen-containing compounds isolated from Sarcodon imbricatus on influenza A virus through regulation of ZBP-1 mediated necroptosis
Year: 2024
Type: article
Abstract: This study focuses on the elucidation of the structure and antiviral properties of six nitrogen-containing compounds including amino acid derivates ( 1 and 2 ) and heterocyclic compounds ( 3–6 ) isolated from the fruiting bodies of Sarcodon imbricatus , particularly Compound 2 , an ( S )-2-(hydroxyimino)-3-methylpentanoic acid ethyl ester. Their antiviral effects were tested against influenza A virus (IAV) in A549 cells. Particularly, Compound 2 exhibited significant antiviral activity in post-treatment assays, reducing viral protein expression and inhibiting viral replication with an IC 50 of 14.9 μmol/L. Additionally, it demonstrated anti-inflammatory effects by reducing levels of cytokines such as TNF-α, IL-6 and IL-1β as well as the oxidative stress induced by IAV infection, while inhibiting necroptosis, a form of programmed cell death associated with inflammation. Thus, our findings demonstrate the antiviral and anti-inflammatory properties of Compound 2 , making it a promising candidate for further research as an anti-influenza agent. • Six nitrogen-containing compounds from Sarcodon imbricatus were isolated. • Compound 2 showed potent antiviral activity against IAV. • Compound 2 regulates ZBP-1 mediated necroptosis signaling pathway such as PIPK1, PIPK3 and MLMK. • Compound 2 inhibited oxidative stress and inflammation response in IAV-infected A549 cells.
Source: Biomedicine & Pharmacotherapy
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,420
Awards: G7039744936, G7613838905
OpenAlex ID: W4405320485