Work

Protocol for preparation of primary alveolar epithelial type I cells from mouse lungs

Year: 2024

Type: article

Abstract: Currently, the only approach for obtaining alveolar epithelial type I (AT1) cells is through the differentiation of cultured AT2 cells, which has several limitations. Here, we present a protocol to prepare AT1 cells directly from mouse lungs. We detail the steps for dissociating epithelial cells from the alveoli, followed by the purification of AT1 cells. Additionally, we describe how to assess the purity of AT1 cells using immunofluorescence staining and flow cytometry, along with approaches for verifying their functions.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2024.103484

Authors: Lulu Huang, Amy Tang, Bojing Shao

Institution: New York Blood Center

Language: English

Topic: Neonatal Respiratory Health Research

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500