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Overexpression of the Global Transcriptional Regulator LaeA Leads to Production of Cyclic Lipopeptides in Marine‐Derived Aspergillus niger L14

Year: 2024

Type: article

Abstract: Overexpression of a global transcriptional regulator LaeA was performed to awaken cryptic biosynthetic gene clusters (BGCs) in a marine sponge-derived fungus, Aspergillus niger L14, to obtain secondary metabolites (SMs), and resulted in the production of five previously undiscovered SMs in the wild-type strain, including three cyclic lipopeptides aspochracin (1), JBIR-15 (2) sclerotiotide C (3), kojic acid (4) and penicillic acid (5). Bioassays revealed that compound 2 displayed promising antimicrobial effects on Candida albicans with a minimum inhibitory concentration value of 32 µg/mL and 4 exhibited significant antioxidant activity with a similar half-maximal inhibitory concentration value (5 µg/mL) to that of ascorbic acid. Transcriptome analysis results indicated that the overexpression of LaeA in strain L14 remarkably enhanced the expression of genes involved in polyketide synthase-non-ribosomal peptide synthetase hybrids and amino acid metabolism, demonstrating an effective approach for the production of cyclic lipopeptides.

Source: Chemistry & Biodiversity

DOI: 10.1002/cbdv.202402704

Authors: Wenjuan Zhou, Mengshi Li, Wei Wang, Xuelian Bai, Huawei Zhang

Institutions: Zhejiang University of Technology, Hangzhou Normal University

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: National Key Research and Development Program of China