Work

Protocol for murine multi-tissue deep immunophenotyping using a 40-color full-spectrum flow cytometry panel

Year: 2024

Type: article

Abstract: The innate and adaptive immune systems, though often studied separately, interact deeply and respond to stimuli simultaneously, with leukocytes displaying a range of pro- to anti-inflammatory phenotypes. This protocol details a procedure for characterizing murine innate and adaptive immune phenotypes using a 40-color full-spectral flow cytometry panel. We describe steps for organ collection, sample preparation, immunofluorescent staining, and acquisition to reproducibly and cost-effectively study tissue-resident leukocytes, their subpopulations, and inflammatory status in various organs.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2024.103492

Authors: Pierre Lemaitre, Chantal Mathieu, Conny Gysemans

Institution: KU Leuven

Language: English

Topic: T-cell and B-cell Immunology

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500