Work

Integrative multi‐omics reveals the mechanism of ulcerative colitis treated with Ma‐Mu‐Ran antidiarrheal capsules

Year: 2024

Type: article

Abstract: Ethnopharmacological relevance: Ma-Mu-Ran Antidiarrheal Capsules (MMRAC) is a traditional Chinese medicine compound formula with anti-inflammatory, antidiarrheal and improving intestinal function.Aim of the study: This study established a ulcerative colitis (UC) mouse model and explored its mechanism of action under MMRAC treatment.Methods: The mechanism of action of MMRAC in the treatment of UC model was investigated by combining metabolomics, transcriptomics and intestinal microbiota detection techniques. Meanwhile, histopathological analysis, serum and colonic detection of inflammatory factors and oxidative stress indicators, Western blotting analysis and PCR analysis and immunofluorescence analysis were performed in order to reveal the mechanism of action of MMRAC on UC treatment.Results: Using pathological changes and biochemical indicators, we constructed a dextrose sodium sulfate-induced UC model in mice and determined the optimal dose of MMRAC. Transcriptome analysis revealed that compared to the control group, 100 genes were upregulated and 744 genes were downregulated in the model group; 360 of these genes underwent changes following MMRAC treatment. Metabolomic analysis using colon tissue yielded 14 colon tissue metabolites with significant differences. We identified three main metabolic pathways and four differential genes in MMRAC-treated UC by integrating transcriptomics and metabolomics data. MMRAC investigated the molecular mechanism of UC treatment and found differences in gene and protein levels in reverse transcription-polymerase chain reaction, western blot, and immunofluorescence. A significant change in the microbiota was seen in dextran sulfate sodium-treated mice. The gut health-associated bacteria genera were shown to be associated with UC, as were changes in their abundance.Conclusion: Based on a thorough multi-omics analysis of transcriptomics, metabolomics, and gut flora, it was determined that MMRAC regulated cysteine and methionine, arginine, and sphingolipid metabolisms and their respective genes BHMT, PHGDH, iNOS, and SPHK1, which in turn served to inhibit UC-generated inflammatory responses and oxidative stress. Additionally, MMRAC regulated the abundance of Coprococcus, Helicobacter, Sutterella, Paraprevotella, and Roseburia in the intestinal tracts of UC mice, which was regulated toward normal levels, thereby restoring normal intestinal function.

Source: Rapid Communications in Mass Spectrometry

DOI: 10.1002/rcm.9939