Verification of the analytical performance of the serum phosphorus assay on Abbott Architect ci8200
Year: 2024
Type: article
Abstract: This study aimed to evaluate the analytical performance of the serum phosphorus assay using the Abbott Architect ci8200® automated system in the biochemistry laboratory of CHU Mohammed VI, Oujda. Method verification was conducted following NF ISO 15189 standards and COFRAC guidelines, assessing key performance parameters such as repeatability, reproducibility, and measurement uncertainty. The colorimetric method for phosphorus determination demonstrated excellent precision, with coefficients of variation (CV) for repeatability ranging from 0.93% to 1.06% across low, medium, and high concentrations. Reproducibility assessments yielded CVs between 2.07% and 2.53%, indicating robust intra-laboratory performance. A comparative analysis between systems using the Bland-Altman method showed strong agreement, with a correlation coefficient of 0.997. Measurement uncertainties at all levels were within acceptable limits. These findings confirm the reliability and accuracy of the assay, highlighting its suitability for clinical diagnostics and its alignment with international accreditation standards.
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W4405356071