Expediency of Studying Endometrium Microbiocenosis in Woman With Infertility in the Program of Preparation for Assisted Reproductive Techologies
Year: 2024
Type: article
Study design: Observational Study
Abstract: Objective – to examine the microbiocenosis of the endometrium in women with infertilityduring EXP preparation for embryo transfer in assisted reproductive technology (ART)programs and to analyze the possible role of infectious agents as one of the potentialfactors in failed ART attempts.Materials and Methods. The study investigated the characteristics of endometrialmicrobiocenosis in 147 women, divided into two groups: the main group (103 patientswith various forms of infertility for whom ART was planned) and the control group(44 practically healthy women of reproductive age who had children and had no issueswith conception). Women in the main group were further divided into two subgroups:I (18 patients with a history of failed ART attempts) and II (85 women undergoing ART forthe first time). In the aforementioned patients, microbiological examination of endometrialbiopsies was performed, which in the main group were obtained during hysteroscopy asa screening procedure in preparation for ART. Results. The study revealed a significantly higher incidence of endometrial contaminationwith pathogenic and opportunistic bacterial flora in the main group (81.6 %), whichexceeded the control group’s rates (10.3 %, p<0.001) by 71.2 %. In the main group,the endometrium was found to harbor both absolute pathogens and opportunisticmicroorganisms, as well as intracellularly persistent pathogens, unlike the results inthe reproductive healthy women, where only opportunistic microflora was detected.It was found that the frequency of endometrial contamination in the main group wasaccompanied by an intensive growth of microorganisms (II-IV degrees of growth),indicating a potential etiopathogenetic effect of the microflora on the development ofpathological processes in the uterine mucosa. Meanwhile, in the control group, therewas only II-degree growth of opportunistic pathogens, suggesting the persistence ofmicroorganisms without triggering inflammation.Conclusions. Including microbiological examination of the endometrium as a standardin the preparation for assisted reproductive technologies will help to identify potentialrisks caused by infectious contamination and improve the effectiveness of these infertilitytreatment methods.
Institution: Bukovinian State Medical University
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4405375810