Fabricating a novel bioinspiring 3D ‐printed sodium alginate/silk fibroin/polyvinyl alcohol/nano‐hydroxyapatite composite scaffold structure for bone tissue engineering application
Year: 2024
Type: article
Abstract: In the present work, we fabricated 3D‐printed biofunctionalized nanocomposite scaffold structures using bioinks prepared by incorporating different concentrations (0.5%–1.5% w/v) of nano‐hydroxyapatite (n‐HA) into optimized a tri‐polymer complex blend comprising of sodium alginate (SA), silk fibroin (SF), polyvinyl alcohol (PVA) with composition represented as SA70/SF30/PVA3, wherein, SA/SF is in 70:30 (v/v) ratio and PVA is in 3% (w/v) ratio. The printed scaffolds consist of microfibers with fiber diameter and pore size range of 437–806 and 389–446 μm having pore interconnecting networks as assessed by scanning electron microscope image analysis. However, with increased n‐HA content, the formation of a microfibrous porous structure with interconnected pores. The amorphous and hydrophilic characteristics of the scaffolds were revealed by XRD analysis and contact angle values ranging from 52 ± 2° to 66 ± 1°. SA70/SF30/PVA3 scaffold containing 1.5% (w/v) n‐HA has shown a controlled degradation pattern. The highest compressive strength of 0.193 MPa was shown by SA70/SF30/PVA3/n‐HA1.5 scaffold. The fabricated scaffolds are cytocompatible, as evident from cell adhesion and MTT assays. Among the scaffolds, the SA70/SF30/PVA3/n‐HA1.5 scaffold exhibited better biological activities as evaluated by an in vitro cell study. A higher alkaline phosphatase activity signifying superior osteogenic performance of the SA70/SF30/PVA3/n‐HA1.5 scaffold could be a favorable substitute for bone tissue engineering applications. Highlights Nano hydroxyapatite provided structural stability to the scaffold. Controlled degradation pattern of the fabricated scaffold. Higher compressive strength of the scaffold. Hydrophilic and amorphous nature of the scaffold. The scaffold has a favorable environment for bone tissue regeneration.
Source: Polymer Engineering and Science
DOI: 10.1002/pen.27014
Institution: National Institute of Technology Rourkela
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W4405377321