Understanding lipidomics associations and the lipoprotein-related caveats in population epidemiology
Year: 2024
Type: article
Study design: Observational Study
Abstract: Mass spectrometry lipidomics is becoming customary to analyze serum/plasma samples in epidemiology. The measurables are molecular constituents of lipoprotein particles, but very little is known about the consequences of adjusting lipidomics data with lipoprotein measures. We studied 2 population cohorts with 5657 and 2036 participants. Liquid chromatography/tandem mass spectrometry lipidomics was applied to analyze 24 molecular lipid classes and nuclear magnetic resonance spectroscopy to quantify 7 lipoprotein lipids plus apolipoprotein A-I and B. The associations of these measures were analyzed via partial Spearman's correlations. The effects of 9 different lipoprotein adjustments on these interrelationships were assessed. Multivariable regression modeling with these adjustments was also performed for the associations between the lipidomics data and body mass index. These novel large-scale lipidomics data and their associations between the lipoprotein measures were coherent in both population cohorts, confirming the compatibility of the analytical approaches. Simulated data were generated to corroborate the mediation effects. The lipoprotein-related lipid transport and metabolism inherently mediate the lipidomics associations, as evident from the striking effects of the lipoprotein adjustments. These effects and their relevance to the interpretations of lipidomics data are presented and discussed in detail for the first time. The combined lipoprotein lipid adjustments appear prone to overadjustment and arbitrary biases.
Source: American Journal of Epidemiology
DOI: 10.1093/aje/kwae445
Institutions: University of Oulu, Finnish Institute for Health and Welfare, MRC Centre for Environment and Health, Imperial College London, Brunel University of London, Tampere University, Tampere University Hospital, University of Tampere, Fimlab (Finland), University of Turku, Turku University Hospital, University of Eastern Finland
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $5,099
Funders: Foundation for Cardiovascular Research, Yrjö Jahnssonin Säätiö, European Commission, Kela, Academy of Finland, Suomen Kulttuurirahasto, Jane ja Aatos Erkon Säätiö, Juho Vainion Säätiö, Signe ja Ane Gyllenbergin Säätiö, Emil Aaltosen Säätiö, Sydäntutkimussäätiö, Tampereen Tuberkuloosisäätiö, Diabetesliitto, Paavo Nurmen Säätiö, Tays, Syöpäsäätiö, Directorate for Biological Sciences, Medical Research Council, European Regional Development Fund, H2020 European Research Council
Awards: G1037428335, HDHL: A precision nutri-epigenetic approach to tackle the mother-to-child transmission of impaired glucose metabolism, G1601895641, G1686171937, G1763104746, G1872575776, Ancestral environmental exposures and offspring health – a multigenerational epidemiologic cohort study across 3 generations, G1891033315, G2026946236, G2230772921, G2330237237, G2333840978, G2513778610, G2641752829, A high-dimensional approach for unwinding immune-metabolic causes of cardiovascular disease-depression multimorbidities, G3111916513, G3213590351, G3297420224, G33539551, G3582688621, G3858258543, G4139205196, G4655867613, G4965406020, G5090981026, G5208171454, G523551622, G5474452856, G5489727732, G5512925732, G5646563246, G5770909501, G6164432047, G6310393984, G692875121, G69797456, Lifelong and transgenerational epigenetic profiles in relation to the development of cardiometabolic diseases, G8646291755
OpenAlex ID: W4405398323