Work

Cannabigerol Treatment Shows Antiproliferative Activity and Causes Apoptosis of Human Colorectal Cancer Cells

Year: 2024

Type: article

Abstract: Objectives: To determine growth inhibitory and anti-cancer effects of Cannabigerol (CBG) in human colorectal cancer cells.Methods: Anti-proliferative effect of CBG was examined using MTT assay and two colorectal cancer cells (SW480 and LoVo cells).Cell death ratio was analyzed using Annexin V/ PI staining experiment.Cell cycle distribution was analyzed using flow cytometry.We also performed western blot analysis on apoptotic marker proteins.Results: CBG showed growth inhibitory effect in colorectal cancer cells using MTT assay.IC 50 concentration of CBG was 34.89 μM in SW480 cells and 23.51 μM in LoVo cells.Annexin V/PI staining showed that CBG treatment increased apoptotic cells from 4.8% to 31.7% in SW480 cells and from 7.7% to 33.9% in LoVo cells.Flow cytometry confirmed that CBG increased sub G 1 population via G 1 arrest in both SW480 and LoVo cells.Western blot analysis showed that CBG increased expression levels of cell death-related proteins such as cleaved PARP-1, cleaved caspase 9, p53, and caspase 3. Conclusion: CBG treatment shows antiproliferative activity and causes apoptosis of colorectal cancer cells, suggesting that CBG is applicable as a promising anticancer drug.

Source: Journal of pharmacopuncture

DOI: 10.3831/kpi.2024.27.4.332