Work

Aglianico grape pomace as a source of antioxidant and anti-proliferative biomolecules: Eco-friendly extraction and HRMS/MS-based molecular networking

Year: 2024

Type: article

Abstract: Grape pomace (GP), a by-product of the wine supply chain process, contains bioactive molecules with known healthy properties. This study examines the impact of different extraction techniques on three GPs of Aglianico cultivar [Cantine del Notaio, Barile, and Torrecuso]. Five eco-friendly extractive techniques [maceration (MAC), digestion (DIG), accelerated solvent extraction (ASE), microwaves (MW), and ultrasound (US)] were used with 50 % ethanol/water as solvent. Spectrophotometric assays showed that DIG and ASE extracts had the highest antioxidant activity and specialized metabolite content. Using the HRMS/MS-based molecular networking, DIG and ASE extract metabolome profiles were analyzed, identifying unknown compounds and known ones with validated antioxidant and chemopreventive effects. In vitro cell-based assay on HepG2 cells demonstrated that Barile GP DIG extract has the highest anti-proliferative activity. Hence, this work provides insight into the potential application of Barile GP DIG extract as a rich source of specialized metabolites with antioxidant and anti-proliferative activity. • Aglianico Grape Pomace (GP): a valuable source of bioactive compounds. • Comparison of green extractive methods on Aglianico GP. • GP extract made at 60 °C showed highest Antiproliferative activity on HepG2. • Aglianico GP extracts not affect cell viability of non-cancerous (IHH) cell line. • Metabolome profile study by HRMS/MS-based molecular networking of Aglianico GP.

Source: Food Chemistry

DOI: 10.1016/j.foodchem.2024.142573