Work

Development of a full field-of-view spectroscopic single-molecule fluorescence instrument with improved methods for calibration

Year: 2024

Type: article

Abstract: We have developed a high-throughput, full field-of-view (FOV) spectroscopic single-molecule fluorescence instrument, overcoming distortion issues with gratings and prisms. Our calibration techniques ensure spectral uniformity across the whole FOV, and our two-step Gaussian fit method enables better than 2 nm precision in spectral peak determination for accurate molecule identification, making it a valuable tool for multi-color single-molecule detection and analysis.

Source: Applied Physics Express

DOI: 10.35848/1882-0786/ada10b

Authors: Deqi Yi, Shangyuan Deng, Chuang Tan, Jie Ma

Institutions: Sun Yat-sen University, State Key Laboratory of Optoelectronic Materials and Technology

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,250

Funder: National Natural Science Foundation of China