Unveiling the promise of peptide nucleic acids as functional linkers for an RNA imaging platform
Year: 2024
Type: article
Abstract: and in live cells, enhancing visualization of RNA in stress granules and U-bodies at low concentrations. The modular nature of the Riboglow platform allows for flexible modifications of the PNA linker, fluorophore, and RNA tag, while maintaining high specificity and affinity. This work establishes a new approach for enhancing RNA imaging platforms through the use of PNA linkers, highlighting the potential of combining short oligonucleotides with small molecules to improve the affinity and specificity of RNA-targeting probes. Furthermore, this dual-binding approach presents a promising strategy for driving advancements in RNA-targeted drug development.
Source: RSC Chemical Biology
DOI: 10.1039/d4cb00274a
Institution: University of Colorado Boulder
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,962
Awards: Riboglow: a robust multi-color riboswitch-based platform for imaging RNA in living cells, Using Riboglow to Define RNP Interactions in Response to Environmental Stress, NIST-CU COOPERATIVE AGREEMENT 2015-2020 (FOR JILA), Riboswitches and their application to RNA visualization and transcription factor interactions with the transcriptome, Acquisition of multi-mode, high-resolution, high-sensitivity imaging platform, G5491260734, G5922074933, G696235137, G718312037, G7932592095, G828594454, G9000046354, G9000059544, G9000085107, G9000118279, G9000194468
OpenAlex ID: W4405569369