Year: 2024
Type: article
Abstract: Skeletal muscle regeneration and functional recovery after minor injuries requires the activation of muscle-resident myogenic muscle stem cells (i.e. satellite cells) and their subsequent differentiation into myoblasts, myocytes, and ultimately myofibers. We recently identified secreted ADAMTS-like 2 (ADAMTSL2) as a pro-myogenic regulator of muscle development, where it promoted myoblast differentiation. Since myoblast differentiation is a key process in skeletal muscle regeneration, we here examined the role of ADAMTSL2 during muscle regeneration after BaCl 2 injury. Specifically, we found that muscle regeneration was delayed after ablation of ADAMTSL2 in myogenic precursor cells and accelerated following injection of pro-myogenic ADAMTSL2 protein domains. Mechanistically, ADAMTSL2 regulated the number of committed myoblasts, which are the precursors for myocytes and regenerating myofibers. Collectively, our data support a role for myoblast-derived ADAMTSL2 as a positive regulator of muscle regeneration and provide a proof-of-concept for potential therapeutic applications.
Source: npj Regenerative Medicine
Institution: Icahn School of Medicine at Mount Sinai
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,890
Awards: G3157465739, Regulation of Skeletal Growth by Soft Tissue Extracellular Matrix, G4706963027, G4780079576, Mechanisms for Regenerative Healing in Intervertebral Discs, G5934968595, G7668312864, G9000029012, Role of TNFalpha in discogenic pain progression and as a treatment target, Engineered Asymmetric Hydrogel for Muscle Stem Cell Polarity and Fate Specification
OpenAlex ID: W4405577413