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Surface Modifications and Antifungal Efficacy of Origanum Oil Incorporation in Denture-based Materials: An In Vitro Study

Year: 2024

Type: article

Abstract: Aim: This study aimed to assess if the addition of origanum oil to denture materials could decrease microorganisms counts and biofilm formation without changing their mechanical/surface properties.Materials and methods: A total of 66 resilient denture liner discs (SoftConfort, Dencril Comércio de Plásticos Ltda, SP, Brazil) were prepared with fixed dimensions of 10 × 3 mm for biofilm assay (n = 36) and 12 × 2 mm for sorption-solubility tests (n = 30) containing three oil concentrations -0, 2.5 and 5%, thereby n = 12 per each group samples for biofilm assay and n = 10 per each group for sorption-solubility test respectively.While the microcosm biofilms of Streptococcus mutans, Candida species, and total microorganisms formed on denture liner were counted and expressed as colony-forming units per disc surface area, the water sorption (WS) and solubility (SL), was calculated by weighing the samples with an analytical balance at different intervals after storing them in a desiccator and distilled water alternatively.Data was recorded and statistically analyzed.Results: Surface roughness increased in all groups after biofilm formation (p < 0.001), with the most significant change observed in the 2.5% oil group.The tested oil concentrations did not result in sorption changes, but a 5% oil concentration resulted in higher solubility (p < 0.001).A reduction of total microorganisms and S. mutans was seen after 24 hours for all concentrations (p < 0.05).No significant reduction was found for Candida (C.) albicans after 24 hours, whilst 2.5% oil concentration presented lower counts of C. albicans in comparison to the 5% group after 24 hours (p < 0.05).Conclusions: Incorporating 2.5% origanum essential oil into resilient denture liners seems to reduce microorganisms count in a complex biofilm model.These results need to be confirmed in future studies. Clinical significance:The addition of natural products like origanum oil into denture-based materials can help manage biofilm onset and development while offering a simple and effective approach for maintaining denture hygiene.This strategy enhances the antimicrobial properties of denture liners without significantly altering their mechanical and surface characteristics, potentially improving patient outcomes.

Source: The Journal of Contemporary Dental Practice

DOI: 10.5005/jp-journals-10024-3760

Authors: Maria ÁL Feitosa, Victório Poletto-Neto, Tamires T Maske, Rogerio A Freitag, Rafael G Lund, Tatiana Pereira-Cenci