Work

Production Biotechnology for a Brucellosis Antigenic Latex Diagnosticum Based on Polyacrolein Microspheres

Year: 2024

Type: article

Abstract: This research was aimed at developing a biotechnology for obtaining a latex brucellosis antigenic diagnosticum based on polyacrolein microspheres. This diagnosticum is intended for detecting antibodies against brucellosis in a latex agglutination reaction in human blood sera. Optimal conditions for latex sensitization with the antigen in the ratio of 1:3 at 45°C for 3 h followed by blocking of active centers with 0.5% gelatin were determined. The developed preparation showed high efficiency, which confirmed its prospects for brucellosis diagnostics. The analytical sensitivity of the diagnosticum was not less than 1:2560, in the absence of cross-reactions with heterologous sera. The diagnostic characteristics of experimental series of the preparation in control testing showed a sensitivity of 80% and a specificity of 93.33%.

Source: Journal Biomed

DOI: 10.33647/2074-5982-20-4-18-26

Authors: M. M. Kurnoskina, I. V. Zharnikova, D. V. Rusanova, A. G. Koshkidko, E. V. Zhdanova, D. G. Ponomarenko

Institution: Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Language: English

Topic: Brucella: diagnosis, epidemiology, treatment

Subfield: Small Animals

Field: Veterinary

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W4405589143

FWCI:

Cites: 4

Cited by:

Related to: 10