Year: 2024
Type: article
Abstract: Hepatitis E virus (HEV) infects humans and several mammals and it has 8 genotypes (HEV1 HEV8). HEV1 4 is the causative agent of Hepatitis E in humans. HEV5 6 was detected only in wild boar in Japan, HEV7 8 was detected in camels. HEV3 4 is characterized by zoonotic potential. Swines and wild boars are main natural reservoirs for this virus. Besides, HEV 3 was detected in deers, dolphins, rabbits, cattle, goats that is additional risk for virus interspecies transmission from domestic animals to humans. Availability of mismatched nucleotides in the complexes of primer/probe with viral targets was applied for estimation of primer sets. Based on determined conserved fragments two sets with LNA containing primer and linear LNA containing probe without mismatched nucleotides in the complexes of primer/probe with single stranded amplicon for real time reverse transcriptase PCR detection of swine and wild boar HEV 3 and HEV 4 isolates were designed. Primer sets may be used for HEV detection by standard PCR
Author: O. Yu. Lymanska
Language: English
Subfield: Hepatology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W4405592201