Work

Sygyzium claviflorum fruit extract preadipocyte differentiation inhibition in 3T3-L1 cells

Year: 2024

Type: article

Abstract: Objective: fruit extract (SCFE) anti-adipogenic attributes in 3T3-L1 cells. Methods: The polyphenolic compounds in SCFE were identified with Reverse phase-high performance liquid chromatography (RP-HPLC). Meanwhile, murine 3T3-L1 preadipocytes, measuring leptin levels, reactive oxygen species (ROS), and lipid and triglyceride (TG) contents were utilized during anti-adipogenic activity assessments. Concurrently, the effects of SCFE on adipogenic transcription factors were established with quantitative real-time-polymerase chain reaction (qRT-PCR). Results: The RP-HPLC results indicated three polyphenolic compounds in SCFE, including one flavonoid (naringin) and two phenolic acids (syringic and p-coumaric). Although SCFE treatments (250-1000 μg/mL) did not result in cell toxicity, they significantly reduced dose-dependent lipid accumulation, ROS production, and TG and leptin levels relative to control-differentiated adipocytes. Moreover, SCFE suppressed sterol regulatory element binding protein-1 (SREBP-1), peroxisome proliferator-activated receptor-gamma (PPAR-γ), and CCAAT/enhancer-binding protein-alpha (C/EBP-α) gene expressions during preadipocyte differentiation into adipocytes. Conclusion: The findings revealed the anti-adipogenic properties of SCFE, indicating its potential as a natural obesity management remedy. Nevertheless, more studies are necessary to elucidate the reactions resulting in SCFE anti-adipogenic effects and the active constituents responsible for the property.

Source: Journal of Taibah University Medical Sciences

DOI: 10.1016/j.jtumed.2024.12.008

Authors: Rozaini M. Zohdi, Muhammad A. Adli, Shahida M. Mukhtar, Awang H. Awang Junaidi, Md Zuki A. Bakar

Institutions: Universiti Teknologi MARA System, Universiti Teknologi MARA, Universiti Putra Malaysia

Language: English

Topic: Adipokines, Inflammation, and Metabolic Diseases

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $625