Year: 2024
Type: article
Abstract: Protein engineering enables the creation of tailor-made proteins for a variety of applications. ImmTACs stand out as promising therapeutics for cancer and other treatments while also presenting unique challenges for stability, formulation, and delivery. We have shown that ImmTACs behave as Janus particles in solution, leading to self-association at low concentrations, even when the average protein-protein interactions suggest that the molecule should be stable. The formation of small but stable oligomers was confirmed by static and dynamic light scattering and analytical ultracentrifugation. Modeling of the structure using AlphaFold leads to a rational explanation for this behavior, consistent with the Janus particle assembly observed for inverse patchy particles.
DOI: 10.1021/jacs.4c08932
Authors: Emily Sakamoto-Rablah, Jordan Bye, Arghya Modak, Andrew Hooker, Shahid Uddin, Jennifer J. McManus
Institutions: University of Bristol, Immunocore (United Kingdom)
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
OpenAlex ID: W4405616337