Work

Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose

Year: 2024

Type: article

Abstract: For studies involving glycosyltransferases and nucleotide sugar transporters, radioactive nucleotide sugars are critical reagents. Of these, GDP-L-[3H]Fucose is currently commercially unavailable. Here, we present a facile approach for the preparation of GDP-[3H]-L-Fucose, using the enzymatic machinery present in the cytosol of the non-infectious and easily cultivated protozoan, Crithidia fasciculata, and its purification by solid phase extraction ion exchange chromatography.

Source: Glycobiology

DOI: 10.1093/glycob/cwae097

Authors: Jose Carlos Paredes Franco, Maria Lucia Sampaio Güther, Michael A J Ferguson

Institutions: University of Notre Dame, University of Dundee, Wellcome Centre for Anti-Infectives Research

Language: English

Topic: Carbohydrate Chemistry and Synthesis

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,691

Funder: Wellcome Trust