Protocol for in vivo recording of neural activity in deep structures of mice brain via gradient lenses by calcium imaging
Year: 2024
Type: article
Abstract: Calcium (Ca 2+ ) imaging is a viable approach for imaging neuronal activity patterns in local brain circuits in living animals. Here, we present a protocol for gradient lens implantation in deep brain structures followed by in vivo Ca 2+ imaging. We describe in detail the steps for surgery preparation, followed by lens implantation, setup for awake head-fixed imaging, and the recording process. For complete details on the use and execution of this protocol, please refer to Cherepanov et al. 1 • Perform GRIN lens implantation for long-term repeatable calcium imaging in awake mice • Image neuronal activity by calcium imaging in head-fixed awake mice • Image local circuits in the arcuate nucleus of the hypothalamus Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Calcium (Ca 2+ ) imaging is a viable approach for imaging neuronal activity patterns in local brain circuits in living animals. Here, we present a protocol for gradient lens implantation in deep brain structures followed by in vivo Ca 2+ imaging. We describe in detail the steps for surgery preparation, followed by lens implantation, setup for awake head-fixed imaging, and the recording process.
Source: STAR Protocols
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,500
Funder: Agence Nationale de la Recherche
OpenAlex ID: W4405638276