Simultaneous screening and quantitation of human milk oligosaccharides by liquid chromatography—Mass spectrometry
Year: 2024
Type: article
Abstract: Human milk oligosaccharides (HMOs) are the third most abundant solid component in human milk (HM) and play a vital role in shaping the gut-microbiota and modulating neonatal immunity. Despite identifying 162 HMOs, clinical research primarily focused on a few. High throughput methods that encompass a broader range of HMOs for clinical studies remain limited. This study aimed to develop a novel method for simultaneously quantifying seven key HMOs (i.e., 2′-fucosyllactose, 3′-sialyllactose, 6′-sialyllactose, lacto-N-tetraose, lacto-N-fucopentaose I, lacto-N-difucohexaose I, disialyllacto-N-tetraose), and screening a broad HMO panel. The method utilizes hydrophilic interaction liquid chromatography coupled to mass spectrometry in polarity switching mode, and MS/MS annotation against the NIST Milk Oligosaccharide MS Library. Analysis of 40 HM samples pre- and post-pasteurization identified 110 HMOs. Additionally, the use of Feature-Based Molecular Networking for novel HMO structure identification is introduced. However, higher MS n fragmentation dimensions and complementary analytical techniques are necessary to enhance annotation confidence, structural assignments, and standardize HMO isolation. These findings highlight the diversity of HMOs and suggest implications for HM banks, where HMO profiles and maternal secretor phenotypes of donors are often unexamined.
Institutions: Universitat de València, Instituto de Investigación Sanitaria La Fe, Hospital Universitari i Politècnic La Fe, Instituto de Salud Carlos III, Red de Salud Materno Infantil y del Desarrollo, Leitat Technological Center, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
Language: English
Topic: Infant Nutrition and Health
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,210
Awards: G1175115578, Fact-based personalised nutrition for the young, G1341303, G1422035950, G210041235, G2262748287, G2531036324, G2601397997, G2672435700, G300979063, G3028028495, G34346518, G3480869486, G3681454997, G4042783231, G425668148, Fact-based personalised nutrition for the young, G434337789, G5080475149, G5195188938, G5709733449, G6296419658, Administración de Vesículas Extracelulares aislados de Leche Materna Donada como suplemento nutricional para la prevención del desarrollo de Enterocolitis Necrotizante en el recién nacido prematuro – estudio en fase I, G699591620, G7084143925, G792975244, G8615950443, G8696694383, G8992583678
OpenAlex ID: W4405650381