N-acetyltransferase 10 regulates UNC-51-like kinase 1 to reduce tubular cell injury and kidney stone formation
Year: 2024
Type: article
Abstract: Objective: Among the most common chronic kidney diseases, kidney stones are second only to hypertension. Kidney stones pose a public health threat due to their increased incidence, high recurrence rate, and heavy economic burden. In this work, we investigated the potential mechanism of N-acetyltransferase 10 (NAT10) in oxidative stress and pyroptosis of renal tubular epithelial cells (RTECs). Material and Methods: A kidney-stone cell model was simulated using calcium oxalate monohydrate (COM) in vitro . Western blot analysis of NAT10 expression and N4-acetylcytidine RNA immunoprecipitation verified the regulatory efficacy of NAT10 in Unc-51 like autophagy activating kinase 1 (ULK1) ac4C modification. The luciferase reporter gene assay further verified the interaction between NAT10 and ULK1. A kidney stone model was established using BALB/c mice injected with glyoxylic acid. Results: COM can dose-dependently suppressed the cell viability and superoxide dismutase activity of HK-2 cells and promoted the release of lactate dehydrogenase and malondialdehyde levels ( P < 0.05). COM also promoted apoptosis in HK-2 cells, upregulated the protein levels of caspase-1 and gasdermin D-N, and simultaneously enhanced the HK-2 cell secretion of interleukin-1b (IL-1b) and IL-18 ( P < 0.05). The overexpression of NAT10 in HK-2 cells reversed the aforementioned effects, and that of NAT10 upregulated the messenger RNA (mRNA) levels of ULK1 and increased ac4C modification ( P < 0.01). Furthermore, only the luciferase activity of the wild-type ULK1 containing NAT10 binding sites was enhanced with the upregulation of NAT10 ( P < 0.001). Actinomycin D treatment showed that NAT10 overexpression extended the half-life of ULK1 mRNA ( P < 0.01). Silencing of ULK1 neutralized the effects of NAT10 overexpression on COM-induced cell injury ( P < 0.05). In addition, the increased expression of NAT10 inhibited crystal deposition, oxidative stress, and apoptosis in vivo ( P < 0.05). Conclusion: This study confirmed that NAT10 inhibits RTECs oxidative stress and cell pyrodeath through the enhanced ac4C modification of ULK1 and impedes kidney stone progression.
Source: CytoJournal
Authors: Le Wang, Jinjing Huang, Lei Song, Ben Ke
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4405654050