Primitive to visceral endoderm maturation is essential for mouse epiblast survival beyond implantation
Year: 2024
Type: article
Abstract: The implantation of the mouse blastocyst initiates a complex sequence of tissue remodeling and cell differentiation events required for morphogenesis, during which the extraembryonic primitive endoderm transitions into the visceral endoderm. Through single-cell RNA sequencing of embryos at embryonic day 5.0, shortly after implantation, we reveal that this transition is driven by dynamic signaling activities, notably the upregulation of BMP signaling and a transient increase in Sox7 expression. Embryos deficient in Hepatocyte nuclear factor-1-beta (Hnf1b−/−), a gene critical for visceral endoderm differentiation, showed an interaction between visceral endoderm and epiblast, crucial for epiblast survival. Single-cell RNA profiling of Hnf1b−/− visceral endoderm shows developmental delays and severe dysregulation in several nutrient transport pathways. Impaired glucose uptake in Hnf1b−/− embryos suggests that the activation of nutrient transport mechanisms during the primitive-to-visceral endoderm transition may be vital for post-implantation epiblast development. These findings offer new insights into the molecular regulation of early mammalian development. • The primitive to visceral endoderm maturation displays dynamic signaling activities • E5.0 exhibits primitive and visceral endoderm transcriptomes at intermediate levels • Hnf1b KO lethality suggests the crucial interaction of epiblast and visceral endoderm Biological sciences; Cell biology; Developmental biology; Embryology; Natural sciences
Source: iScience
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,150
OpenAlex ID: W4405695377