Year: 2024
Type: article
Abstract: Commonly used adenine-induced chronic kidney disease (CKD) murine models often employ C57BL/6 mice; however, this strain has inherent limitations due to its natural resistance to developing key pathological features of CKD, such as tubulointerstitial fibrosis and inflammation. There have been attempts to overcome these barriers by using multiple concentrations of adenine-supplemented diets or by performing prolonged experiments up to 20 weeks. Here, we demonstrate that SKH1 Elite mice develop clinically relevant CKD phenotypes (e.g., polyuria, proteinuria, inflammation, and renal fibrosis) over the course of only 6 weeks of low-dose (0.15%) adenine supplementation. As a docile, immunocompetent, and hairless strain, SKH1 Elite mice offer several logistical advantages over C57BL/6 mice, including ease of handling and the ability to study dermal conditions, which are often secondary to CKD.
Source: Cells
Authors: Benjamin W. French, Joshua D. Breidenbach, Shereen G. Yassine, Bella Z. Khatib-Shahidi, Sara Kazmi, Caitlin M. Murphy, Humza S. Bashir, Evan M. Benson, Bivek Timalsina, Upasana Shrestha, Dhilhani Faleel, Satkeerth Boyapalli, Prabhatchandra Dube, Apurva Lad, Irum Syed, Deepak Malhotra, Amira Gohara, David J. Kennedy, Steven T. Haller
Institutions: University of Toledo, Los Alamos National Laboratory
Language: English
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,069
Awards: G1252839233, G1829728128, G215843557, Lake Erie Center for the Great Lakes and Human Health, G6005809994, G6232862013, G6286020778, G6352879310, G6777394803, Lake Erie Center for Fresh Waters and Human Health, Inhalation Exposure to Aerosolized Cyanotoxins Worsens Pre-Existing Asthma via Activation of Granulocytic Inflammation, G7955816292, G8167257546, Counter regulatory mechanisms of cardiotonic steroids in cardio-renal syndrome, G9000027421, G9000185647, G9000339728
OpenAlex ID: W4405699693