Photoacoustic and fluorescence dual-modality imaging of cerebral biomarkers in Alzheimer’s disease rodent model
Year: 2024
Type: article
Abstract: SignificanceAlzheimer’s disease (AD) is a predominant form of dementia that can lead to a decline in the quality of life and mortality. The understanding of the pathological changes requires monitoring of multiple cerebral biomarkers simultaneously with high resolution. Photoacoustic microscopy resolves single capillaries, allowing investigations into the most affected types of vessels. Combined with confocal fluorescence microscopy, the relationship between plaque deposition and small vessel pathology could be better understood.AimWe aim to introduce a dual-modality imaging system combining photoacoustic microscopy (PAM) and confocal fluorescence microscopy (CFM) to provide a comprehensive view of both cerebral cortical vessels and amyloid-β (Aβ) plaque in AD mouse model in vivo and to identify the pathological changes of these two biomarkers.ApproachWe developed a dual-modality imaging system to image both cerebral vessel structure and Aβ plaque on groups of mice with different ages and phenotypes. Vessel imaging is enabled by PAM, whereas Aβ plaque is imaged by CFM with the aid of fluorescent dye.ResultsThe small vessel density in the AD group was significantly lower than in the control group, whereas the Aβ plaque density in the AD group was not only higher but also increased with age.ConclusionsThis dual-modality system provides a powerful platform for biomarker monitoring of AD expressing multi-dimensional pathological changes.
Source: Journal of Biomedical Optics
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,675
Funders: International Retinal Research Foundation, Research to Prevent Blindness, Ohio State University, University of Michigan, Cardiff University, Macquarie University, Sun Yat-sen University, Umeå Universitet, Peking Union Medical College, National Institutes of Health, University of California, Los Angeles, National Institute on Aging, National Heart, Lung, and Blood Institute, National Eye Institute, National Institute of Neurological Disorders and Stroke, Alcon Research Institute
Awards: G1038665822, G2026354636, G2200624748, G245191160, G3131410269, Wearable real-time functional brain mapping for a non-human primate stroke model, G3463356597, G491460452, G667672624, G7715434445, Multimodal Molecular Imaging of Choroidal Neovascularization, G9000010830, G9000138755, Unraveling the role of tPA in the neurovascular unit
OpenAlex ID: W4405702287