Year: 2024
Type: article
Abstract: Helical aromatic oligoamide foldamers bearing anionic side chains that mimic the overall shape and charge surface distribution of DNA were synthesized. Their interactions with chromosomal protein Sac7d, a non-sequence-selective DNA-binder that kinks DNA, were investigated by Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Circular Dichroism spectroscopy (CD), melting curve analysis, Atomic Force Microscopy (AFM), and Nuclear Magnetic Resonance (NMR), as well as by single crystal X-ray crystallography. The foldamers were shown to bind to Sac7d better than a DNA duplex of comparable length. The interaction is diastereoselective and takes place at the DNA binding site. Crystallography revealed that the DNA mimic foldamers have a binding mode of their own and that they can bind to Sac7d without being kinked.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $5,510
Funders: Australian Government, European Commission, Deutscher Akademischer Austauschdienst, Deutsche Forschungsgemeinschaft, European Synchrotron Radiation Facility, Institut National de la Santé et de la Recherche Médicale, China Scholarship Council, Centre National de la Recherche Scientifique, Hubei University, HORIZON EUROPE Framework Programme, European Research Council, Deutsches Elektronen-Synchrotron, HORIZON EUROPE European Research Council
OpenAlex ID: W4405706096