Year: 2024
Type: article
Abstract: We report the development of a small molecule-based barcoding platform for pooled screening of nanoparticle delivery. Using aryl halide-based tags (halocodes), we achieve high-sensitivity detection via gas chromatography coupled with mass spectrometry or electron capture. This enables barcoding and tracking of nanoparticles with minimal halocode concentrations and without altering their physicochemical properties. To demonstrate the utility of our platform for pooled screening, we synthesized a halocoded library of polylactide-co-glycolide (PLGA) nanoparticles and quantified uptake in ovarian cancer cells in a pooled manner. Our findings correlate with conventional fluorescence-based assays. Additionally, we demonstrate the potential of halocodes for spatial mapping of nanoparticles using mass spectrometry imaging (MSI). Halocoding presents an accessible and modular nanoparticle screening platform capable of quantifying delivery of pooled nanocarrier libraries in a range of biological settings.
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $5,510
Funders: National Science Foundation, University of Minnesota, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Materials Research Science and Engineering Center, Harvard University, National Cancer Institute, National Institute of Biomedical Imaging and Bioengineering, Division of Materials Research, Division of Electrical, Communications and Cyber Systems
Awards: G1801249269, G2360436881, G259601046, G310967942, G3274162344, G3618943016, G403942265, G4470453470, G4774430914, Understanding drug delivery through an integrated barcoding approach, G5108852245, G6995829561, G743970376, G7573957695, University of Minnesota Materials Research Science and Engineering Center, NNCI: Midwest Nano Infrastructure Corridor (MINIC), Cancer Center Support (Core) Grant, G941982536
OpenAlex ID: W4405708158