Electrochemical Magnetic Immunoassay for the Determination of the Fish Allergen β-Parvalbumin
Year: 2024
Type: article
Abstract: β-parvalbumin (β-PV) is the primary fish allergen responsible for most allergic reactions in individuals sensitive to fish. To ensure food safety, a sandwich-based magnetic immunoassay was developed using maleimide-functionalized magnetic beads (NH-MBs). Specific anti-β-PV antibodies were immobilized on these MBs, and a screen-printed carbon electrode was employed as the electrochemical transducer. A linear concentration range from 10 to 1000 ng/mL, a limit of detection of 1.8 ng/mL, and a limit of quantification of 7.1 ng/mL were achieved. Nineteen commercial food samples were analyzed to assess the potential of the sensor for routine use in food quality control. Important factors such as protein source and food processing (e.g., boiling, grilling, and frying) and preservation (e.g., in oil, and vacuum) were evaluated. The validated results confer the usefulness of the assay for food quality control.
Source: Biosensors
DOI: 10.3390/bios14120639
Language: English
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,069
Awards: G1240268737, Sensores eletroquímicos para a deteção e quantificação de vestígios de alergénios em produtos alimentares, G2346795057, Laboratório Associado para a Química Verde - Tecnologias e Processos Limpos, Laboratório Associado para a Química Verde - Tecnologias e Processos Limpos, G2756637201, G2814222956, G2975900969, G4823118686, G5454278309, G6997658380, Development of analytical strategies for screening and sample preparation for tetrodotoxin analysis, Laboratório Associado para a Química Verde - Tecnologias e Processos Limpos, G8963217260
OpenAlex ID: W4405721685