Work

Bioinspired design of DNA in aqueous ionic liquid media for sustainable packaging of horseradish peroxidase under biotic stress

Year: 2024

Type: article

Abstract: We show that a combination of DNA and ionic liquid significantly increases the stability and activity of HRP and achieves a 4.8-fold higher peroxidase activity than PBS buffer. Also, HRP retains 84% of its activity in IL+DNA compared to 24% in PBS against trypsin digestion. Molecular modeling and spectroscopic studies reveal a protective microenvironment.

Source: Chemical Communications

DOI: 10.1039/d4cc05803h

Authors: Diksha Dhiman, Aaftaab Sethi, Rakesh Sinha, Sagar Biswas, Gregory Franklin, Dibyendu Mondal

Institutions: Institute of Plant Genetics, Polish Academy of Sciences, Polish Academy of Sciences, Adam Mickiewicz University in Poznań, Jain University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: Narodowe Centrum Nauki, Horizon 2020

Awards: G3661635942, G8092314748