Work

Evaluation of a commercial multiplex real-time PCR panel for identification environmental water antibiotic-resistant bacteria in hemoculture bottles

Year: 2024

Type: article

Abstract: Background: Antimicrobial resistance (AMR) is a major public health problem worldwide, and it is increasing, especially in developed and developing countries, including Thailand. Sepsis is a crisis and emergency causing the death of patients with a high mortality rate of 1 in 5 of the global population. Using inappropriate antimicrobial drugs to treat infections increases the chance of drug resistance in the microorganisms, and precise and rapid treatment is essential for patient management. Objective: This cooperative study aims to evaluate a commercial multiplex real-time PCR panel, QIAstat-Dx BCID GN Plus AMR Panel, to detect antimicrobial-resistant genes in the bloodstream. Materials and methods: Thirty bacterial samples were used to simulate bloodstream infections. The comparison between the QIAstat-Dx BCID GN Plus AMR Panel and the conventional PCR method was revealed. Results: Both methods gave concordant results for 12 samples, while results for 15 were discordant. Three samples could not be detected using the QIAstat-Dx BCID GN Plus AMR Panel because ebc gene is not included. The QIAstat-Dx BCID GN Plus AMR Panel revealed a sensitivity, specificity, positive predictive value, and negative predictive value of 91.50%, 93.30%, 74.10%, and 98.50%, respectively. Conclusion: The study indicated that the QIAstat-Dx BCID GN Plus AMR Panel Cartridge can detect common antimicrobial-resistant genes from the bloodstream. However, additional resistant genes should be included in the panel to cover various highly prevalent antimicrobial-resistant genes based on geographical location.

Source: Journal of Associated Medical Sciences

DOI: 10.12982/jams.2025.029

Authors: Pimwan Thongdee, Putt Narongdej, Passara Wongthai, Sirachat Nitchaphanit, Kasidach Chuenchom, Kanlaya Krataipech, Jantima Raksatham, Dutsadee Peerapongpaisarn, Wilawan Oransathit, Sirigade Ruekit, Nattaya Ruamsap, Piraporn Juntanawiwat, Paphavee Lertsethtakarn, Pongthorn Narongroeknawin, Tanit Boonsiri

Institutions: Phramongkutklao College of Medicine, King Mongkut's Institute of Technology Ladkrabang, Armed Forces Research Institute of Medical Science, Walter Reed Army Institute of Research, Phramongkutklao Hospital

Language: English

Topic: Bacterial Identification and Susceptibility Testing

Subfield: Clinical Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W4405729403

FWCI:

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