Work

Probable New Species of Bacteria of the Genus Pseudomonas Accelerates and Enhances the Destruction of Perfluorocarboxylic Acids

Year: 2024

Type: article

Abstract: Bacteria of the genus Pseudomonas are the most studied microorganisms in the biodestruction of persistent perfluoroorganic pollutants, and the research on their application for the remediation of environmental sites using biotechnological methods remains relevant. The aim of this study was to investigate the ability of a known destructor of perfluorooctane sulfonic acid from the genus Pseudomonas to accelerate and enhance the depth of destruction of long-chain perfluorocarboxylic acids (PFCAs), specifically perfluorooctanoic acid and perfluorononanoic acid, in water and soil in association with the strain P. mosselii 5(3), which has previously confirmed genetic potential for degrading PFCAs. The complete genome (length 5.86 million base pairs) of the strain 2,4-D, a new species of Pseudomonas, was sequenced, assembled, and analyzed. The genomes of both strains contain genes involved in the defluorination of fluorinated compounds, including haloacetate dehalogenase H-1 (dehH1) and haloalkane dehalogenase (dhaA). The strain 2,4-D also has a multi-component enzyme system consisting of a dioxygenase component, an electron carrier, and 2-halobenzoate 1,2-dioxygenase (CbdA) with a preference for fluorides. The strain 2,4-D was able to completely decarboxylate and defluorinate PFCAs in an aqueous cultivation system within 7 days, using them as the sole source of carbon and energy, but it only converted them to perfluorheptanoic acid. However, it assisted strain 5(3) (which defluorinated them during the same period to perfluorohexanoic acid) in converting PFCAs to perfluoropentanoic acid, accelerating the process by 24 hours. The degradation and transformation of the anionic acids were assessed using LC-MS analysis, and the release of indicators for mineralization of fluoride ions was determined by ion chromatography. In a model experiment for bioaugmentation of microorganisms in artificially contaminated soil, the degradation of PFCAs by the association of pseudomonads also occurred faster and deeper than by the individual strains, achieving a degree of biodestruction of 75% over 60 days, with perfluoropentanoic acid being the main metabolite of destruction. Thus, it has been demonstrated that strains of Pseudomonas bacteria with genetic potential for the biodegradation of PFCAs are capable of realizing this potential in association, exhibiting an additive effect.

Source: Toxics

DOI: 10.3390/toxics12120930

Authors: Sergey Chetverikov, Gaisar Hkudaigulov, Danil Sharipov, Sergey Starikov

Institutions: Russian Academy of Sciences, Institute of Biology Ufa Science Center

Language: English

Topic: Per- and polyfluoroalkyl substances research

Subfield: Environmental Chemistry

Field: Environmental Science

Domain: Physical Sciences

SDG: Clean water and sanitation

Open Access status: gold

Estimated APC paid: $2,955