Work

Taiyintiaowei-Tang ameliorates hepatic steatosis of mice fed with acute high-fat diet plus alcohol binge by blockade of NLRP3 inflammasome

Year: 2024

Type: article

Abstract: Taiyintiaowei-Tang (TYTWT), a traditional Chinese Korean medicine formula, has been demonstrated to ameliorate hepatic lipid accumulation. However, the precise mechanism remains uncertain. Hepatic steatosis is classified into alcoholic and non-alcoholic forms while alcohol consumption exacerbates non-alcoholic liver disease. Considering the therapeutic effect of TYTWT on liver function, the study aimed to investigate the underlying mechanism of TYTWT on the comorbid of alcoholic and non-alcoholic hepatosteatosis. A mouse model of comorbid alcoholic and non-alcoholic hepatosteatosis was established by feeding with high-fat diets (HFD) plus alcohol binge. In addition, to simulate the microenvironment that hepatocytes encounter, HepG2 cells and AML12 cells were stimulated by oleic acid (OA), palmitic acid (PA) and alcohol in vitro . TYTWT improved the alteration of biochemical indexes in serum induced by HFD plus alcohol binge and reduced the expression of lipid accumulation-related protein sterol regulatory element binding transcription factor 1 (Srebp1) and inflammatory proteins including cysteinyl aspartate specific proteinase-1 (Caspase-1), purinergic receptor P2X, ligand-gated ion channel 7 (P2x7r), apoptosis-associated speck-like protein containing CARD (Asc) and Il1b at mRNA level in mice livers. Further, TYTWT inhibited the expression of SREBP1, P2X7R, CASPASE-1, IL-1β and NLR family and pyrin domain containing3 (NLRP3) in OA, PA or alcohol-stimulated HepG2 cells. Notably, TYTWT prevented lipogenesis in OA, PA or alcohol-stimulated HepG2 cells and AML 12 cells. TYTWT significantly attenuated hepatic lipid accumulation caused by HFD feeding plus alcohol binge and inhibited the inflammatory response of hepatocytes through P2X7R-NLRP3 axis, thus reversing the hepatic steatosis.

Source: Journal of Traditional and Complementary Medicine

DOI: 10.1016/j.jtcme.2024.12.003