Automation and miniaturization of high-throughput qPCR for gene expression profiling
Year: 2024
Type: article
Abstract: method. Successful amplification was observed for the reference samples when using FR and 1.5x conditions. The 2.5x condition exhibited suboptimal amplification with a lower success rate while the 5x condition retrieved no amplification. The 2.5x and 5x miniaturization conditions were excluded from further runs. A strong significant positive correlation was observed between the manual and automated workflows for the reference RNA sample, underscoring the robustness of the gene expression assay. The automation of the immunology and pregnancy gene expression panels on the 45 individual samples retrieved a success rate >70 % for both the FR and the 1.5x miniaturization conditions. A significant positive correlation was also observed between the FR and 1.5x miniaturization conditions for both panels. Our results show that the adoption and the 1.5x miniaturization capabilities of Mosquito HV system for automating the gene expression workflow did not interfere with data quality and reproducibility.
Source: SLAS TECHNOLOGY
Institutions: Sidra Medical and Research Center, Saveetha University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,800
OpenAlex ID: W4405751425