Work

Counter-diffusion studies of human transthyretin: the growth of high-quality crystals for X-ray and neutron crystallography

Year: 2024

Type: article

Abstract: A crystallogenesis study of human transthyretin using the counter-diffusion method is described as an alternative to conventional convective vapour diffusion and batch approaches for protein crystallization. The X-ray diffraction results show systematic trends that exhibit unique patterns of crystallization and high crystal quality as well as a remarkable degree of coherence within extended crystal rods that wholly fill the capillaries used. Preliminary neutron diffraction data have been recorded from a number of these samples, validating the feasibility of this methodology for neutron crystallography.

Source: Journal of Applied Crystallography

DOI: 10.1107/s1600576724011191

Authors: Clare De'Ath, Mizar F. Oliva, Martine Moulin, Matthew P. Blakeley, Michael Haertlein, Edward P. Mitchell, José A. Gavira, Matthew W. Bowler, V. Trevor Forsyth

Institutions: European Synchrotron Radiation Facility, Institut Laue-Langevin, Instituto Andaluz de Ciencias de la Tierra, European Molecular Biology Laboratory, Lund University

Language: English

Topic: Enzyme Structure and Function

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,870

Funders: Université Grenoble Alpes, Liam M. Kinne Foundation, Human Frontier Science Program, Agence Nationale de la Recherche, European Synchrotron Radiation Facility, Vetenskapsrådet, Ministerio de Ciencia e Innovación, French Infrastructure for Integrated Structural Biology, Erasmus+

Awards: G103193202, CBH-EUR-GS, Transthyretin amyloidosis and therapy: bridging the gap between laboratory and clinical research, G3692898361, G4876571084, Infrastructure Française pour la Biologie Structurale Intégrée

OpenAlex ID: W4405757581

FWCI: 0.095

Cites: 73

Cited by: 1

Related to: 10