Syringin alleviates ROS-induced acute lung injury by activating SIRT1/STAT6 signaling pathway to inhibit ferroptosis
Year: 2024
Type: article
Abstract: Acute lung injury (ALI) is the critical respiratory condition. Syringin with anti-inflammatory and anti-oxidant properties can exhibit the lung protective effects. SIRT1 and STAT6 can exert protective roles against lung injury by inhibiting ferroptosis. In the current study, A549 lung epithelial cells were treated with 200 μM H 2 O 2 for 2 h to establish an in vitro ALI model. Then, H 2 O 2 -stimulated A549 cells were treated with syringin to identify the biological role of syringin in ROS-induced ALI. Moreover, H 2 O 2 -stimulated A549 cells were further treated with SIRT1 inhibitor EX527 or ferroptosis activator erastin to elucidate whether syringin could exert protective effects against ROS-induced ALI depending on SIRT1/STAT6 signaling-mediated ferroptosis inhibition. It was verified that syringin treatment improved the impaired viability and mitigated inflammatory response and oxidative stress of H 2 O 2 -stimulated A549 lung epithelial cells by activating SIRT1/STAT6 signaling pathway. Syringin treatment inhibited the ferroptosis of H 2 O 2 -stimulated A549 lung epithelial cells by activating SIRT1/STAT6 signaling pathway. Treatment with SIRT1 inhibitor EX527 or ferroptosis activator erastin both reversed the alleviating effect of syringin on H 2 O 2 -induced A549 lung epithelial cell injury. To sum up, syringin treatment alleviates H 2 O 2 -induced lung epithelial cell injury by activating SIRT1/STAT6 signaling pathway to inhibit ferroptosis. • Syringin activates SIRT1/STAT6 pathway in H 2 O 2 -stimulated lung epithelial cells. • Syringin inhibits the ferroptosis of H 2 O 2 -stimulated lung epithelial cells by activating SIRT1/STAT6 pathway. • Syringin alleviates H 2 O 2 -induced lung epithelial cell injury by activating SIRT1/STAT6 pathway to inhibit ferroptosis.
Source: Tissue and Cell
Authors: Xuemei Cai, Yanan Wu, Fuxia Liu, Jinping He, Yanhua Bi
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $2,780
OpenAlex ID: W4405761217