The Ability of Nano Chitosan and Bacillus subtilis (Ehrenberg) Cohn to Suppress the Growth of Alternaria porri (Ellis) Ciferri
Year: 2024
Type: article
Abstract: Purple blotch disease, caused by the fungus Alternaria porri (Ellis) Ciferri, poses a significant threat to shallot crops. Environmentally friendly control methods, such as applying nano-sized chitosan and Bacillus subtilis (Ehrenberg) Cohn, offer promising alternatives. This study aims to determine the effective concentrations of nano chitosan and B. subtilis , individually and in combination, to suppress the growth of A. porri in-vitro . The research was conducted from March to June 2024 at the Phytopathology Laboratory and Plant Protection Biotechnology Laboratory, Department of Pests and Plant Diseases, Faculty of Agriculture, Universitas Padjadjaran. A completely randomized design was employed, consisting of nine treatments with three replications each: a control treatment, B . subtilis 10 7 CFU/ml, 50 ppm nano chitosan, 100 ppm nano chitosan, 200 ppm nano chitosan, B . subtilis 10 7 CFU/ml + 50 ppm nano chitosan, B . subtilis 10 7 CFU/ml + 100 ppm nano chitosan, B . subtilis 10 7 CFU/ml + 200 ppm nano chitosan, and 80% mancozeb. The results indicated that nano chitosan at a concentration of 100 ppm was the most effective single treatment which could suppress the growth of A . porri colonies by 94% and inhibit conidia germination by 95%. Meanwhile, B . subtilis 10 7 CFU/ml + 100 ppm nano chitosan is the most effective mixed treatment which could suppress the growth of A . porri colonies by 84% and inhibit conidia germination by 90%.
Authors: Muhammad Restu Al Farisy, Rika Meliansyah, Agus Susanto, Hersanti Hersanti
Institution: Padjadjaran University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W4405765001