Work

FITA-Containing 2,4-Dinitrophenyl Alkylthioether-Based Probe for Detection and Imaging of GSH

Year: 2024

Type: article

Abstract: Glutathione (GSH) plays a crucial role in various physiological processes and its imbalances are closely related to various pathological conditions. Probes for detection and imaging of GSH are not only useful for understanding GSH chemical biology but are also important for exploring potential theranostic agents. Herein, we report a fast intramolecular thiol-activated arylselenoamides (FITA)-based fluorescent probe using 2,4-dinitrophenyl alkylthioether as a sulfydryl-selective receptor for the first time. The fluorescence of the probe was low due to the double effects of PET, while the probe exhibits an 86-fold fluorescence enhancement at 460 nm after GSH activation and a detection limit of 0.95 μM. Furthermore, the probe is low-toxic and capable of imaging cellular GSH. This work further expands the design and applicability of the FITA-based platform, offering a new thiol-deprotection strategy for development of fluorescent probes.

Source: Sensors

DOI: 10.3390/s25010034

Authors: Yalun Dong, Liyue Wang, Wenfang Liang, Jiqin Zhu, Lu Sun, Long Yi

Institutions: Beijing University of Chemical Technology, Tianjin Medical University

Language: English

Topic: Sulfur Compounds in Biology

Subfield: Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,955

Funders: National Natural Science Foundation of China, Beijing University of Chemical Technology, Tianjin Medical University